Neural Signaling | News & Updates

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IH) and immunocytochemistry (IC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IH and IC.

Biotinylated neurotrophins: These labeled neurotrophins can be used with any antibody to obtain highly specific localization.

Click here to view our complete line of immuno-colocalization products.

Fixed brain sections were incubated with human BDNF-Biotin (#B-250-B) followed by Streptavidin-Cy3 (red). Same sections were incubated with Anti-TrkB (extracellular)-ATTO-488 Antibody (#ANT-019-AG), (green).

Products for Live Cell Flow Cytometry

^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Ephrin-A2 (extracellular) Antibody(#AER-032), 5 µg + goat-anti-rabbit-FITC.

^___ Unstained cells + goat-anti-rabbit-AlexaFluor-647 secondary antibody.
^___ Cells + Anti-Ret (extracellular) Antibody(#ANT-025), (1:20) + goat-anti-rabbit-AlexaFluor-647 secondary antibody.

Alomone Labs is committed to developing cutting edge reagents for visualizing neurotrophin receptors in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various receptors. Below are new antibodies ideal for LCI.

Click here to view our complete line of LCI products.

Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. Extracellular staining of cells using Anti-Neuropilin-1 (extracellular) Antibody (#ANR-063), (1:25).

With the many roles allotted to proBDNF, we have developed a novel tool for studying and visualizing the protein in living cells. human proBDNF-Biotin (#B-256-B) is highly purified human proBDNF (cleavage resistant) (#B-256), conjugated to biotin.

Quantum-dot conjugated to human proBDNF-Biotin (#B-256-B) (0.25 µg/ml), was added to the distal side of spinal cord explants grown for few days in microfluidic chamber, followed by axonal live imaging using a spinning disc confocal microscope.